84-1082-95　NaDC-3 (Sodium Dicarboxylate Transporter) 100ul　N0016

特徴

• The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity.
• Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys.
• The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues.
• These are NADC1and NADC3 (NADC2 is found in C.
• Elegans).
• NADC1 is Na+-coupled, electrogenic, and exhibits low affinity for its dicarboxylatesubstrates.
• This isoform is expressedprimarily in the brush border membrane of intestinal and renalepithelial cells.
• The physiological function of NADC1 is to absorbthe intermediates of the citric acid cycle, such as citrate, succinate,-ketoglutarate, fumarate, and malate, in the intestine and kidney.NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibitsrelatively higher affinity for its substrates compared with NADC1.
• The NADC3is expressed primarily in the basolateral membrane of intestinaland renal epithelial cells.
• However, it is also found in tissuessuch as liver, placenta, and brain.
• NADC3 in the kidney is involvedin generating the driving force for the organic anion transporterOAT1 to facilitate the active entry of organic anions into thetubular cells across the basolateral membrane.
• In the brain,NADC3 mediates the cellular uptake of N-acetylaspartate, a processclosely linked to myelination.
• Therefore, the physiologicalfunctions of the NADCs may extend beyond the mediation of cellularentry of citric acid cycle intermediates.
• Recently, a third member of this familyin mammals has been identified, Na+-coupled citrate transporter (NACT), mediates the cellular uptakeof citrate in a Na+-coupled manner.
• NADC-3, (SCL13A3 gene) a sodium dependent high- affinity dicarboxylate transporter with 600aa each in mouse and rat, it's a 602aa sequence in human ( chr 20q12-q13.1) expressed in kidney, brain, liver and placenta.
• NADC-3 exhibits 48% identity with NADC-1.
• The transport function of NADC-3 shows a sigmoidal relationship with regard to Na+ concentration, NADC3 accepts a number of dicarboxylates including dimethyl-succinate as substrates and excludes monocarboxylates.
• Transport of succinate is markedly influenced by pH, the transport gradually decreases when pH is acidic.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See N0016B
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.