84-1082-94　［取扱停止］NaDC-1 (Sodium Dicarboxylate Transporter) 100ug　N0015D

特徴

• The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity.
• Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys.
• The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues.
• These are NADC1and NADC3 (NADC2 is found in C.
• Elegans).
• NADC1 is Na+-coupled, electrogenic, and exhibits low affinity for its dicarboxylatesubstrates.
• This isoform is expressedprimarily in the brush border membrane of intestinal and renalepithelial cells.
• The physiological function of NADC1 is to absorbthe intermediates of the citric acid cycle, such as citrate, succinate,-ketoglutarate, fumarate, and malate, in the intestine and kidney.NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibitsrelatively higher affinity for its substrates compared with NADC1.
• The NADC3is expressed primarily in the basolateral membrane of intestinaland renal epithelial cells.
• However, it is also found in tissuessuch as liver, placenta, and brain.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique
• ELISA: 0.5-1ug/ml; N0015E Control peptide can be used to coat ELISA plates at 1ug/ml.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.