The sodium-dicarboxylate cotransporters (Solute carrier family 13) , which transport succinate and other Krebs cycle intermediates, fall into 2 categories based on their substrate affinity, low affinity and high affinity.
Both the low- and high-affinity transporters play an important role in the handling of citrate by the kidneys.
The two different Na+-coupled dicarboxylate transporters (NADC) have been identified in mammalian tissues.
These are NADC1and NADC3 (NADC2 is found in C.
Elegans).
NADC1 is Na+-coupled, electrogenic and exhibits low affinity for its dicarboxylatesubstrates.
This isoform is expressed primarily in the brush border membrane of intestinal and renalepithelial cells.
The physiological function of NADC1 is to absorb the intermediates of the citric acid cycle, such as citrate, succinate, ketoglutarate, fumarate and malate, in the intestine and kidney.
NADC3 is also a Na+-coupled and electrogenic dicarboxylate transporter, but it exhibits relatively higher affinity for its substrates compared with NADC1.
NADC3 is expressed primarily in the basolateral membrane of intestinal and renal epithelial cells.
However, it is also found in tissues such as liver, placenta and brain.
Recently, a third member of this familyin mammals has been identified, Na+-coupled citrate transporter (NACT), mediates the cellular uptakeof citrate in a Na+-coupled manner.
NACT, is 568aa protein in human and 572aa in rat.
NaCT is expressed in liver, testis and brain in rat and shows preference for citrate over dicarboxylates; it may play a role in cellular utilization of citrate in blood for the synthesis of fatty acids and cholesterol and for the generation of energy.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using ECL
ELISA: 0.5-1ug/ml; N0007-75A Control peptide can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA.
Specificity：Recognizes rat NACT.
Isotype：IgG1
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to16aa near the C-terminal of rat NACT (KLH). Species Sequence Homology: human, 86%
