Na+/ H+ exchanger regulatory factor (NHERF1) is a PDZ domain containing adaptor protein known to bind to various receptors, channels, cytoskeletal elements and cytoplasmic signaling proteins.
A 358aa mainly expressed in kidney, liver and pancrease by NHERF gene (chrm 17).
And is represented in another isoform, NHERF2.
NHERF1 protein contains 2 tandem PDZ domains of approximately 90aa and a C-terminal sequence that binds several members of the ERM (ezrin-radixin-moesin) family of membrane cytoskeletal adapters.
The protein plays an important role in regulation of NHE3, turnover of G-protein coupled receptors, platelet derived growth factor receptor and ion transporters such as CFTR, Na/Pi cotransporter, NaHCO3 cotransporter and Trp channels.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
Use control peptide at 1ug/ml to coat ELISA plates
Western Blot: 1-10ug/ml using ECL
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human Merlin 1. Species sequence homology: 100% mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：15-aa peptide from Human Merlin1~C-terminus
