84-1082-87　Luteinizing Hormone Releasing Hormone (LHRH) 100ul　L7510-02

特徴

• Human reproduction is regulated by Luteinizing hormone releasing hormone (LHRH), also termed gonadotropin releasing hormone (GnRH).
• It is produced by hypothalamic neurons, secreted in a pulsatile manner into the capillary plexus of the median eminence and effects the release of luteinizing hormone and follicle stimulating hormone from gonadotropic cells in the anterior pituitary.
• The peptide may also influences other activities including behavioral ones, as LHRH or LHRH-like immunoreactivity has been found in gonadal tissue, placenta and the central nervous system, and exogenously administered LHRH is shown to affect behavior.
• LHRH gene (chromosome 8q21) code for a protein of 92 amino acids (rat 92 aa and mouse 90 aa) in which the LHRH decapeptide is preceded by a signal peptide of 23 amino acids and followed by a Gly-Lys-Arg sequence, as expected for enzymatic cleavage of the decapeptide from its precursor and amidation of the carboxy-terminal of LHRH.
• In addition to the LHRH decapeptide, the prohormone contains a 56-amino acid peptide sequence which has been designated gonadotropin-releasing hormone associated peptide (GAP).
• Applications:Suitable for use in ELISA.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See L7510-02B
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.