84-1082-85　［取扱停止］KCC4 (K, Cl Cotransporter 4) 100ug　K0135-01

特徴

• Chloride is a critical component of all living cells.
• It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions.
• Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content.
• The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.
• The K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes.
• This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia.
• The net direction of this transport is out of the cells.
• At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized.
• KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini.
• Most recently, KCC4 (mouse and human 1083 aa) have been cloned that are 65-71% identical to KCC1/KCC2.
• It is highly expressed in heart and kidney.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence technique
• ELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well (K0135-01B)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.