84-1082-84　Insulin-Like Growth Factor Binding Protein 3 (IGFBP3, tcag7.703, BP-53; IBP3, IGF-binding protein 3, Growth hormone-dependent binding protein) 100ul　I7661-16H2

特徴

• The insulin like growth factors (IGFs) are the major growth-promoting factors in the plasma.
• IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation.
• The A and B domains of IGFs are identical to insulin.
• IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity.
• The IGF-IIR lacks the kinase activity and is actually identical to the mannose-6-phophate receptor.
• Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs).
• At least 6 related IGFBPs (IGFBP1-6) have been well characterized.
• Recently, IGFBP-7/Mac25/prostacyclin-stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges.
• These proteins are present in plasma in high concentration as compared to the membrane IGFRs.
• Therefore, IGFBPs have the potential to modulate the IGF action.
• IGFBPs have been shown to either inhibit or stimulate the IGF effects.
• The primary structures of mammalian IGFBPs appear to contain three distinct domains of roughly equivalent sizes: the conserved N-terminal domain, the highly variable mid region, and the conserved C-terminal domain.
• Human IGFBPs share approximately 36% identity.
• Recently several groups of cysteine-rich proteins with discrete, but striking, structural and functional similarities to the IGFBPs.
• This has led to the proposal of an IGFBP superfamily, comprised of the IGFBPs and these IGFBP-related proteins (IGFBP-rP1-9 or IGFBP-8-12). 
• Applications:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000 using Chemiluminescence technique.
• IGFBP-3 is ~40kD.  Concentrated T98G cell serum free culture medium or other cells can be used as positive control
• ELISA: 1:10,000Immunoprecipitation: 5-10ul antiserum per 500ul of conc.
• serum free medium
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.