The insulin like growth factors (IGFs) are the major growth-promoting factors in the plasma.
IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation.
The A and B domains of IGFs are identical to insulin.
IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity.
The IGF-IIR lacks the kinase activity and is actually identical to the mannose-6-phophate receptor.
Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs).
At least 6 related IGFBPs (IGFBP1-6) have been well characterized.
Recently, IGFBP-7/Mac25/prostacyclin-stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges.
These proteins are present in plasma in high concentration as compared to the membrane IGFRs.
Therefore, IGFBPs have the potential to modulate the IGF action.
Applications:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
Concentrated T98 cell serum free culture medium or other cells can be used as positive control
ELISA: 1:10,000 using 50-100ng control peptide/well
Immunoprecipitation: 5-10ul per 500ul of concentrated serum free medium
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes bovine IGFBP2. Species Crossreactivity: human, mouse, rat and primate. Minimal crossreactivity <0.1% with IGFBP-1, -4, -5, and IGFBP-3, <0.5%.
