Rubber (cis-1,4-polyisoprene), an isoprenoid polymer, is produced in about 2000 plant species.
It is the raw material of choice for gloves, tiresand other industrial products.
Allergy to natural rubber latex products has been recognized as a serious medical problem especially among health care workers and children with spina bifida.
Interestingly, there appears to be significant clinical and immunochemical cross-reactivity between some latex proteins and allergens in certain fruits and vegetables, such as banana, kiwi, avocado, and potato, and patients with fruit and vegetable allergy may be at increased risk for reacting to latex proteins.
Proteins leached from the gloves or extractable latex proteins, ranging in size from 5-200kD, have been shown to be involved in eliciting type I hypersensitivity.
Hevea brasiliensis has been the only commercial sourceof natural rubber mainly because of its abundance in the tree,its quality, and the ease of harvesting.
Latex is produced in laticifers, which are specialized structures that consist of anastamosed latex-producing cells.
Harvested Hevea latex is a complete cytosol with high protein content.
Several potential allergens have been identified in Hevea latex, including rubber elongation factor (REF or Hev b 1), hevamine-1,3-glucanase (Hev b 2), hevein preprotein (HEV1), a 24-kD rubber particle-associated protein (RPP) (Hev b 3), and a component of the microhelix protein complex (Hev b 4), a 16kD acidic protein Hev b 5, Hev b 6, and Hev b 7.
Hevein is a chitin-binding protein of 43 amino acids found in the lutoid body-enriched fraction of rubber tree latex.
A hevein cDNA clone (HEV1) encodes a putative signal sequence of 17 amino acids followed by a polypeptide of 187 amino acids.
Interestingly, this polypeptide has two distinct domains: an amino-terminal domain of 43 amino acids, corresponding to mature hevein, and a carboxyl-terminal domain of 144 amino acids.
Hevein is unusually rich in cysteine and glycine.
It has been shown to inhibit the growth of several chitin-containing fungi.
Many chitin-binding proteins contain 1-3 hevein like domains (HLD) arrange in tandem.
Two Wound-induced genes (WIN1 and WIN2) from potato encode proteins with HLD located at the N-terminus.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1: 50,000; Control peptide can be used to coat ELISA plates at 1ug/ml.
See H2034-80B
Western Blot: 1:1000-1:5000 (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes Hevein.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：43aa peptide corresponding to hevein was synthesized, coupled to KLH. Peptide is 100% conserved in Hevein, and prohevein. It is 88% homologous in pseudo-Hevein, 75% in WIN2, 70% in class I Chitinase, and WIN.
