coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues.
Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification.
In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene.
Proteins are expressed as fusion proteins.
Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification.
Therefore, fusion-tags serve as universal tags much like secondary antibodies.
Many tags have their own characteristics.
Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP.
GST-fusion proteins can bind to glutathione-Sepharose.
Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step.
Purity of fusion proteins can be followed by Tag-antibodies.
Very often, fusion proteins are directly injected into animals to generate antibodies.
Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1ug/ml using 50-100ng of H1847-52L, Control Peptide/well
Western Blot: 1-2ug/ml using Chemiluminescence technique
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Conjugated to horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
仕様
Size：100ug
Host：goat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Hemagglutinin Tag.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：A 9aa peptide sequence corresponding to aa114-122, YPY DVP DYA of hemagglutinin influenza virus.
