84-1082-73　HE2, alpha 100ul　H1827-98

特徴

• HE2 is a human epididymis specific sperm binding secretory protein expressed by SPAG11 gene (chromosome 8p23-p22), a 103aa protein adopts a typical antigenic pattern with a subacrosomal equatorial distribution on the sperm head, this localization of HE2 with Beta-defensins in the epithelial defense system of epididymal duct have developed mechanism to fight ascending infections, including local production of these antimicrobial peptides and proteins.
• The regionalized epididymal expression of the HE2 and its isoforms suggests that they exert their effects at different times in the controlled sequence of sperm modification, maturation, binding and a direct role in fertilization.
• HE2 represents 3 major isoforms, HE2-alpha, HE2-beta and HE2-gamma, all variants have a common 71 residue N terminus but various C termini, these variations at C termini is due to frame shifting deletions.
• Thus, identical sequences and deletion, start and stop points indicate that HE2 isoforms are derived from alternative splicing of 8 or more exons of a single gene and working towards common microbial defense and sperm maturation to acquire fertilizing ability.
• The human HE2 protein and its isoforms are 28% conserved.
• A gene product of rat epididymal duct, BIN1B, has been suggested to represent the rat counterpart of human HE2 protein.
• As HE2, BIN1B also shows structural characteristics of beta-defensins.
• HE2 alpha: It appears in two isoforms, HE2 A1 (103aa) and HE2 A2 (70aa).
• HE2 alpha represents major peptide isoforms in human epididymis, processed at the peptide level and secreted apically into the duct lumen, HE2 A2 sequence is entirely different from defensins and from any other known peptide.
• Epididymis restricted expression and sperm binding properties of HE2 alpha suggests its involvement in the process of post-testicular sperm maturation.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using ECL technique
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• See H1827-98A
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.