Glyceraldehyde-3-phosphate dehydrogenase,G3PDH/GAPD/G3PD (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).
G3PDH is a cytosolic enzyme.
It is thought to be a tetramer of identical chains ~36kD.
G3PDH (mouse/rat/rabbit 333; human 335-aa,chromosome 12p13.31-p13.1).
The enzyme is present in such widely separated forms as man, lobster, and E.
coli.
Its rate of evolutionary change is one of the slowest known.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: Control antigen can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：goat
Source Antibody：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes rabbit G3PDH. May crossreact with the G3PDH from other species.
