GST’s are responsible for the metabolism of a broad range of xenobiotics and carcinogens.
The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation.
GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases.
GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes.
Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma.
GSTs exists as homodimers of ~25kD.
GST A1-1 is mainly found in kidney, intestine, lung and liver.
GST M1-1 is restricted to liver and few other tissues.
GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver.
The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
The GST M1 along with GST M2-GST M5 are mapped to Chrm 1, There is a close physical proximity between GSTM1 and GSTM2 loci, which shares 99% nucleotide sequence identity over 460 nucleotides of 3-prime untranslated mRNA.
The 181aa long GST M1 is mainly expressed in liver, brain, testis, kidney and lung and show enzyme activity towards CDNB; AFB1-epoxide; trans-4-phenyl-3buten-2-one.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng/well GST-coated ELISA plates
Western Blot: 1:500-1:3000 (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes rat GSTmu. Likely to crossreact with GSTmu from other species (mouse, human, etc.). Does not crossreact with other GST isoforms (alpha and pi).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Full length rat GSTmu/GST M1-1 fusion protein with N-terminal His-tag (25kD) expressed in E. coli.
