coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues.
Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification.
In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene.
Proteins are expressed as fusion proteins.
Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification.
Therefore, fusion-tags serve as universal tags much like secondary antibodies.
Many tags have their own characteristics.
Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP.
GST-fusion proteins can bind to glutathione-Sepharose.
Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step.
Purity of fusion proteins can be followed by Tag-antibodies.
Very often, fusion proteins are directly injected into animals to generate antibodies.
Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.
Applications:Can be used for immunoprecipitation and for affinity purification of fusion proteins containing the tag.
The column has a binding capacity of approx.
5 mg/ml of beads.
Recommended Dilution:Immunoprecipitation: 25-50ul of gel volume (50-100ul of 1:1 suspension) per 500ug of the protein lysate.
For recombinant proteins with high level of expression, the lysate amounts must be optimized
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store at 4°C.
Do not freeze.
仕様
Size：500ul
Host：goat
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion-exchange chromatography prior to conjugation.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Coupled to agarose at 2-5mg/ml of beads.
Specificity：Recognizes Glutathione S Transferase.
Isotype：IgG
Calc Applications Abbrev：IP
Immunogen：Purified bacterial GST (Schistosoma japonicum, ~27kD), expressed in E. coli.
