84-1082-50　Glutamic Acid Decarboxylase (GAD) 100ul　G7116-01

特徴

• g-Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter.
• The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD; L-glutamate 1-carboxy-lyase, EC 4.1.115).
• In the CNS GAB is entirely restricted to GABAergic neurons.
• GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus.
• Cloning of GAD genes have identified two subtypes: GAD65 (65kD; human 585 AA chromosome 10) and GAD67 (67kD; human 594 AA, chromosome 2) share approx.
• 65% amino acid homology.
• The N-terminus is the most divergent while the C-terminus is highly conserved.
• Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5'-phosphate suggesting their activities are differentially regulated.
• GAD67 is cytosolic, while GAD65 is membrane associated.
• GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain.
• Applications:Western Blot: 1-10ug/ml using Chemiluminescence technique
• ELISA: 1:10,000-1:100,000; using 50-100ng control peptide/well.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.