Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, Mol.
Wt.
40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and c-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting
Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml
Immunohistochemistry: 2-20ug/mlOptimal dilutions to be determined by the researcher.
Control Peptide:G3900-24H: GLUT 3, Human, Control Peptide (Glucose Transporter)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Specificity：Recognizes human GLUT 3. No significant homology is detected with other species. Sequence Homology: 92% with GT14 (duplicon of GT3) short and long form.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa sequence from human GLUT 3. Epitope: ~N-terminus, Extracellular domain
