Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters.
Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, Mol.
Wt.
40-60kD) and Sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD).
Individual member of this family have identical predicted secondary structures with 12 transmembrane domains.
Both N and c-termini are predicted to be cytoplasmic.
Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting
Glut isoform differ in their tissue expression, substrate specificity and kinetic characteristics.
Glut-1 mediates glucose transport into red cells, and throughout the blood brain barrier, and supply glucose to most cells.
Glut-2 provides glucose to the liver and pancreatic cells.
Glut-3 is the main transporter in neurons, whereas Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin.
Glut-5 transports fructose in intestine and testis.
Glut-6/SLC2A6, originally described as glut-9 has been reassigned as Glut-6.
Glut-6 (human 507 aa; ~ 48kD; chromosome 9q34) is most closely related to Glut-8 (~45% homology).
It is highly expressed in brain, spleen, and peripheral leukocytes.
Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane.
Glut-8 is found in adult testis and placenta.
Human Glut-9 is expressed in spleen, peripheral leukocytes and brain.
Human Glut-10 (541 aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility.
It is widely expressed with highest levels in liver and pancreas.
Glut-11 (496 aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle.
Glut-12 (human 617 aa, , monkey 621 aa;~ 50kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue, and small intestine .
glut 13 or proton myo-inositol transporter (HMIT; human 629 aa, rat 618 aa, ~75-90kD/67kD protein) is highly expressed in glial cells and some neurons.
Glut-13 transport activity was specific for myo-inositol.
Rat HMIT is ~35% identical to rat GlutX1.
Applications:Western Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml (See G3900-02A).
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human GLUT 1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：15aa peptide from human GLUT 1. Location: ~N-terminus, Extracellular. Species Sequence Homology: Rabbit - 86%; Porcine, rat - 80%; Bovine, mouse - 73%.
