Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide.
The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2.
GIP, Gastric inhibitory polypeptide, also known as glucose-dependent insulinotropic polypeptide (GIP), is a 42-amino acid hormone (chr 17q21.3) that stimulates insulin secretion in the presence of glucose.
GIP is derived by proteolytic processing of a 153-residue precursor, preproGIP; it is a member of a family of structurally related hormones that includes secretin, glucagon, vasoactive intestinal peptide, and growth hormone-releasing factor.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-10ug/ml using Chemiluminescence technique
ELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C after receipt.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:Recognizes human Gastric Inhibitory Peptide.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to 10aa at the middle region, cytoplasmic domain of human GIP. Species Sequence Homology: mouse, porcine and rat - 90%.