84-1082-39　［取扱停止］β-Galactosidase (E. coli) 100ul　G1041-05F

特徴

• Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices.
• Therefore, tags enable a selective identification and purification of the protein of interest.
• Eukaryotic genes are often cloned into E.
• coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3).
• Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation.
• Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6).
• Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein.
• Applications:Western Blot: 1:1000-1:5000ELISA: 1:1000-1:10,000
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.