Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices.
Therefore, tags enable a selective identification and purification of the protein of interest.
Eukaryotic genes are often cloned into E.
coli B-galactosidase (lacZ) gene, resulting in the expression of a desired protein as a fusion hybrid with B-galactosidase (1-3).
Anti-B-galactosidase antibodies allow a simple isolation of fusion proteins directly from the crude bacterial lysates, using immunoaffinity chromatography (1-5) or used in immunoprecipitation.
Anti-Beta galactosidase can also be used for the immunocytochemical detection of B-galactosidase in cells and tissues that express transfected bacterial lacZ gene (6).
Ant-beta galactosidase may be used in various immunoassays to identify the expression of beta-galactosidase fusion protein.
FITC to Protein Ratio:3-4 FITC per IgG molecule
Applications:Immunofluorescence: 1:100-1:1000.
Do not store diluted (working) solution.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：E. coli
Grade：Highly Purified
Purity：Purified by ion exchange chromatography prior to conjugation.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes b-Galactosidase from E. coli.
Isotype：IgG
Calc Applications Abbrev：IF
Calc Crossreactivity：Ec
Immunogen：Recombinant purified E. coli b-Galactosidase. (~16kD)
