84-1082-33　GABA Receptor A, gamma2 (Gamma-aminobutyric Acid Receptor Type A gamma Subunit, GABRA) 100ul　G1015-20D

特徴

• GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
• GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
• A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric?) protein complexes.
• Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
• GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
• The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality.
• GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1).
• GAA in the brain are the targets of many clinically important drugs
• Human GAG2 protein is 466aa (mouse 466/474aa; human 467aa).
• Applications:Western Blot: 1-2ug/ml using ECL technique
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.