84-1082-32　GABA Receptor A, delta (Gamma-aminobutyric Acid Receptor Type A delta Subunit, GABRAd) 100ul　G1015-19N1

特徴

• GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
• GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
• A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric?) protein complexes.
• Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
• GABA A (GAA) receptor proteins (450-627 aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
• The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality.
• GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1).
• GAA in the brain are the targets of many clinically important drugs.
• Mouse and rat GABA A delta protein is 449aa (Human 452-aa, chromosome 1p36.3).
• Applications:ELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 0.5-1ug/ml (See G1015-19N2)
• Western Blot: 1:1000-1:5000 using ECL technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.