GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain.
GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs).
A family of GABA-A receptors subtypes exists, which are generated by alternative splicing of alpha 1-6, beta 1-4, gamma 1-4, delta, epsilon, pie, theta, and rho1-3 to form a heteromeric (pentameric?) protein complexes.
Various GABA-A subunits show distinct patterns of temporal and spatial expression that may imply its tissue specific physiological role (1).
GABA A (GAA) receptor proteins (450-627aa) are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 3 TM (transmembrane) domains, a large cytoplasmic domain followed by TM4 and C-terminal extracellular domain.
The regions between TM3-4 and the large cytoplasmic loop are least conserved among various GAA subunits, which may confer subunit specific functionality.
GAA genes are distributed as clusters throughout the human genome (chromosomes 4, 5, 15, and X; delta subunit on chromosome 1).
GAA in the brain are the targets of many clinically important drugs
Human GAA1 (chromosome 5q34-q35) protein is 456aa (rat/mouse 455aa). Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME), a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks.
Applications:Western Blot: 1-5ug/ml using ECL technique
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes bovine, human and murine GAA 166-269aa sequences.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Hu Mo
Immunogen：Recombinant Human GAA1 fragment (166-269aa). Location: ~Extracellular domain.
