A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC).
Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins.
Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1).
The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc
Folate Transporter/ FOLT/ RFC, a 512aa each protein in mouse and rat, 591aa in human (chrm 21q22.3, gene SLC19A1) transports folate compounds into mammalian cells via receptor mediated or carrier mediated mechanisms, as such maintains the intracellular concentration of folate.
It shows 65% identity to mouse and hamster folate transporters.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml (See F5753-40A).
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a PBS solution containing 0.05% sodium azide. Reconstitute with 100ul PBS.
Specificity：Recognizes human RFC1.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated 20-amino acid peptide from the N-terminal, cytoplasmic domain of human RFC1. Species Sequence Homology: mouse and rat: 40-45%
