Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2.
Prostaglandin PGE2is one of the major prostaglandin produced during inflammation.
A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described.
The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs.
There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue.
The intracellular signaling also differs among the receptor subtypes.
In general, EP receptors display a protein topology typical of GPCR-7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus.
EP3 (mouse 3-4 isoforms, 365-425 aa; rat 2 isoforms 364-365 aa; human 3-6 isoforms, 365-425 aa, chromosome 1p31.2,) is expressed in the kidney, brain, stomach, uterus, liver, skeletal muscle, intestine, and thymus.
Alternative splicing of EP3 produces various isoforms (alpha, beta, gamma, etc) with different C-terminal tails.
EP3 isoforms have identical ligand binding but different coupling properties with G-proteins: alpha and beta coupled to Gi, whereas gamma couples with Gi and Gs.
EP3 knockout mice show impaired febrile response to pyrogens.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
See E3364-34
Western Blot: 1-10ug/ml using ECL technique.
An antibody made to this epitope has been shown to detect ~ 62kD protein in the kidney (2)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes rat EP3. Rat control peptide is conserved in all EP3 isoforms that differ at the C-terminus. It is 75% conserved in mouse EP3-beta and –gamma. No significant homology of control peptide exists with either human EP3 or other EP (1, 2 or 4) receptors.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rt
Immunogen：15aa peptide of rat EP3, conjugated to KLH. Location: ~N-terminal, Extracellular.
