In the eukaryotic cell, DNA primase initiates DNA replication by the synthesis of small ribonucleotides called primers.
The eukaryotic primase is composed of two subunits, p49 (human 420aa; chromosome 12q13) and p58 (human 509aa, chromosome 6p12-p11.1), which purify as a complex tightly bound to DNA polymerase alpha.
Primase initiates synthesis with a triphosphate purine moiety at the 5'-end.
After synthesis of 7-10 ribonucleotides, the primer-template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit.
The p49 subunit of DNA primase contains the catalytic active site.
Residue 104-111 are most critical for primer synthesis.
Alanine substitution in residues Glu, Asp, and these residues may form part of a conserved carboxylic triad also observed in the active sites of DNA polymerases and reverse transcriptases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cells or other tumor cells
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.5,, 0.2% BSA 0.05% sodium azide before the addition of glycerol to 40%.
Specificity：Recognizes the mouse p49 subunit at ~49kD. No reactivity is seen with p58 subunit. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Purified mouse recombinant DNA primase, p49.
