84-1082-15　Cytochrome C 100ul　C9095-05C

特徴

• Cytochrome C enzyme is a nuclear encoded protein located in the mitochondria of all aerobic cells.
• It is involved in the electron transport system that functions in oxidative phosphorylation.
• It accepts electrons from cytochrome b and transfers them to cytochrome oxidase.
• In the process the iron of the heme group (which is identical to that of hemoglobin and myoglobin) shifts from the ferrous to the ferric state.
• Human cytochrome c has 105aa (~12kD).
• Mitochondria play a pivotal role in the regulation of programmed cell death or apoptosis.
• Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine aspartase).
• The initiator caspases can be activated by self-cleavage.
• The effector caspases are then activated in an amplification cascade.
• Several key participants are released from the mitochondria that regulate apoptosis.
• The first such factor (Cytochrome C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1.
• Binding of the mitochondria factor allows Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9.
• Active caspase-9 then turns on downstream effector caspases, initiating apoptosis.
• Murine embryos devoid of cytochrome c died in utero by midgestation.
• In contrast, cells lacking cytochrome c demonstrated increased sensitivity to cell death signals triggered by TNF.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:3000; Western Blot Positive Control available.
• See C9095-05D.
• Load 10ul per lane
• ELISA: 1:10,000-1:50,000Optimal dilution determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.