84-1082-13　CUG-Binding Protein 2 (NAPOR) 100ul　C7948-50D

特徴

• RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein productsfrom a single structural gene.
• Substitutional RNA editing employsa variety of genetic mechanisms, the biochemical basis of whichhas been elucidated following the development of in vitro assaysthat recapitulate important elements of this process.
• There are two typesof substitutional RNA exist in mammals, namely A-to-I and C-to-URNA editing.
• The best-characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal apolipoprotein B(apo B)).
• Apo B RNA editing changes a CAA to a UAA stop codon,generating a truncated protein, apoB48.
• The functional complex includes a minimal corecomposed of apobec-1 and ACF, that functionas an adaptor protein by binding both the deaminase and the RNAsubstrate.
• The RNA binding proteins also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2)
• CUGBP2/ NAPOR/ Brunol 3/ ETR-3, a novel RNA-binding protein (54kD) found as a component of holoenzyme Apolipoprotein B (apo B) beside apobec-1 and ACF.
• It plays a role in apo B mRNA editing by forming a regulatory complex with the three components of the minimal editing enzyme, apobec-1, ACF and apo B RNA.
• Posttranscriptional C to U RNA editing of apo B creates an in-frame stop codon in the edited transcript that in turn results in translation of a truncated protein apo B48.
• Apo B mRNA editing takes place in mammalian small intestine, it is accordingly an important physiological role in mammalian lipoprotein metabolism.
• The CUGBP2/ NAPOR is a 509aa long protein (Chr 10p13-p14) exists in three isoforms NAPOR 1, 2 and 3.
• NAPOR 1 and 2 are same except additional 19aa residues upstream of NAPOR 1, whereas NAPOR 3 differs from other two because of its 5 extra amino residues upstream of N-terminus of NAPOR 2, also 6aa between its second and third RNA binding domains are absent from NAPOR 3.
• NAPOR 3 is neuron specific in expression whereas the other two are ubiquitous.
• Applications:Western Blot: 1-10ug/ml using ECL technique
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.