84-1082-09　COX 2 (Cyclooxygenase 2, PGHS 2, Prostaglandin Endoperoxide Synthase 2, PHS 2) 100ul　C7904-80H

特徴

• Prostaglandins are important regulators of immune responses, fever, and pain.
• Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 (also called PGHS-1; PHS-1; Prostaglandin-endoperoxide synthase-1) and COX2 (also called PGHS-2; Prostaglandin-endoproxide synthase-2 and PHSII).
• Both forms of COX proteins are membrane associated heme proteins containing Cyclooxygenase and peroxidase activities.
• These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin.
• Cox-1 (human 599 aa; rat 602 aa) is homodimer of 70KD subunits (1).
• COX1 is constitutively expressed although significant enhancement of COX1 expression can be induced in some cell types.
• High expression is observed in gastrointestinal tissues.
• COX2 is a 72KD protein having 60% homology to COX1.
• It is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-100,000 Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Positive Control:3T3 cells, BMMC, L-929, PC-12, C127 and CEF-147.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.