84-1082-06　Core Binding Factor alpha 1 (CBFA1) 100ul　C7900-71B

特徴

• BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton.
• The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and –II receptors.
• The primary unit of bone formation is osteoblast, the bone forming cell.
• These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns.
• Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function
• Cbfa1 / Runx2 / OSF2 (alternatively spliced form 507aa and 522aa in human (chr 6p21) and 528aa or 596aa in mouse) is expressed in bone thymus, testis but not in brain, lung, heart or kidney.
• It is a transcription factor involved in osteoblastic differentiation and skeletal morphogenesis, essential for the maturation of osteoblasts and both intramembranous and endochondral ossification.
• Defects in Cbfa1 are the cause of cleidocranial dysplasia (CCD), an autosomal dominant skeletal disorder with high penetrance and variable expressivity.
• It is due to defective endochondral and intramembranous bone formation.
• Applications:Western Blot: 1-10ug/ml using ECL technique
• ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.