Ceruloplasmin (Cp) is a plasma metalloprotein synthesized and secreted by the liver.
Cp (also known as ferroxidase; iron (II):oxygen oxidoreductase, EC 1.16.3.1) is a blue alpha-2-glycoprotein that binds 90 to 95% of plasma copper and has 6 or 7 cupric ions per molecule.
It is involved in peroxidation of Fe(II) transferrin to form Fe(III) transferrin.
Human Cp is a single polypeptide chain (1065 aa, 135kD; chromosome 8q21.13-q23.1) structurally similar to factors V and VIII.
Cp also has abundant expression in specific populations of glial cells within the brain.
Hereditary Cp deficiency have profound iron accumulation in most tissues.
Cp -/- mice showed no abnormalities in cellular iron uptake but a striking impairment in the movement of iron out of reticuloendothelial cells and hepatocytes.
Cp may play an essential physiologic role in determining the rate of iron efflux from cells with mobilizable iron stores.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100,000; using 50-100ng control antigen/well.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes human Ceruloplasmin at ~135kD.
