84-1081-99　Caspase 11 (Casp4, Caspase 4, Aoptosis-related cysteine peptidase, Casp11, ASP-4, Caspase-4, Caspl, Ich3, ich-3, Protease ICH-3) 100ug　C2088-56E

特徴

• Caspase-11, (also known as CASP-11, ICH-3, ICE-B) promotes IL-b processing by procaspase-1 (ICE) and therefore may also have a role in inflammatory response as evident by a 30 fold increase in CASP-11 activity by LPS.
• The mouse and rat CASP-11 (373aa) are most homologous to human CASP-4 (377aa) protein (ICErel-II, TX, ICH-2) and could possibly be the ortholog of human CASP-4.
• The pro-or zymogen form of CASP-11 is activated by Granzyme-B or cathepsin-B and the processed enzyme consists of two large p20 (20kD) and two small ~p10 (10kDa subunits.
• CASP-11 is highly expressed in lung and spleen, weaker in heart and very little in liver, kidney, testis and skeletal muscle but has not been detected in the brain.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-10ug/ml (ECL)ELISA: 0.5-1ug/ml.
• Control peptide (Cat #C2088-56F) can be used to coat ELISA plates at 1ug/ml.
• Optimal dilution determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.