84-1081-91　Caspase 8 (FLICE, CASP-8, FLICE, ICE-like apoptotic protease 5, MACH, MCH5) 100ul　C2087-42C

特徴

• Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc.
• Apoptosis is driven by specialized proteases known as caspases.
• After the initial discovery of the first mammalian caspase 1 or ICE (interleukin 1 beta converting enzyme), a growing family of caspases 1-14 have been cloned and characterized.
• Caspases are synthesized as inactive zymogen or proenzyme forms (30-55kD), which upon apoptotic stimulation are proteolytically processed (self or by other proteins) in a sequential manner into their active heterotetrameric forms.
• The processed form consists of large subunit (17-20kD) and a small (10-12kD) subunits, which may associate to form an active enzyme.
• Functionally active caspases initiate a proteolytic cascade, capable of cleaving and activating numerous cellular targets including PARP, G4-GDI, DFF, MEKK, etc.
• On a functional basis, two categories of caspases have been defined: the initiator caspases (caspases-8, -9, and –10) are activated in the earlier phases of apoptosis, whereas the executioner caspases (caspases-3, -6, and –7) are activated by initiator caspases and are responsible for dismantling cellular components.
• Caspases are widely distributed in various tissues and cells.
• Caspase-8, also known as (MACH, FADD-like ICE, FLICE, Mch-5, CAP4, MACH-alpha 1) human proenzyme is 479 aa (mouse 480 aa, rat 482 aa).
• It is the most upstream protease of the activation of caspases responsible for CD95 and TNFR-1 induced cell death.
• It cleaves and activates caspases-3, -4, -6, -7, -9, and –10.
• It is a heterodimer of a 19kD (p18) and 10kD (p10) subunits.
• Caspase-8 is alternatively spliced into at least 8 isoforms (1-4 alpha, 1-4 beta).
• Alpha 1 and beta-1 are expressed in a many tissues.
• Highest expression is observed in peripheral blood, leukocytes, spleen, thymus and liver.
• Lower expression is seen in brain and testis.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blotting (1:1K-5K for neat serum and 1-10ug/ml for affinity pure antibody using ECL technique).
• Caspase pro-enzyme is ~55kD that is processed to generate heterodimer of p18 and p10/12.
• ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1ug/ml for affinity pure)
• Optimal dilution determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.