84-1081-88　Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) 100ul　C2087-01D

特徴

• Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc.
• Apoptosis is driven by specialized proteases known as caspases.
• After the initial discovery of the first mammalian caspase 1 or ICE (interleukin 1 beta converting enzyme), a growing family of caspases 1-14 have been cloned and characterized.
• Caspases are synthesized as inactive zymogen or proenzyme forms (30-55kD), which upon apoptotic stimulation are proteolytically processed (self or by other proteins) in a sequential manner into their active heterotetrameric forms.
• The processed form consists of large subunit (17-20kD) and a small (10-12kD) subunits, which may associate to form an active enzyme.
• Functionally active caspases initiate a proteolytic cascade, capable of cleaving and activating numerous cellular targets including PARP, G4-GDI, DFF, MEKK, etc.
• On a functional basis, two categories of caspases have been defined: the initiator caspases (caspases-8, -9, and –10) are activated in the earlier phases of apoptosis, whereas the executioner caspases (caspases-3, -6, and –7) are activated by initiator caspases and are responsible for dismantling cellular components.
• Caspase-3, (also known as CPP32, APOPAIN, PARP CLEAVAGE PROTEASE, CASP-3) mapped at human chromosome 4Q35 is a cytoplasmic enzyme which proteolytically cleaves poly (ADP-ribose) polymerase (PARP), cleaves and activates sterol regulatory element binding proteins (SREBPs), Casp-6, -7 and –9.
• Caspase-3 is also involved in amyloid-beta 4A precursor protein (APP) cleavage in dying neurons of Alzheimer disease.
• Mouse/rat/human Casp-3 (277aa) is composed of two subunits of ~17 and 12kD derived from a 45kD proenzyme CPP32.
• Human Caspase-3 is expressed in larger amounts in lung, spleen, heart, liver and kidney.
• moderate levels in brain and skeletal muscle, and low in testis.
• CASP3s (182aa), a splice variant lacking the 95 residues from the c-term of Casp-3 has been reported.
• Mouse Casp-3 is expressed in heart, brain, liver and muscle but not in kidney or testis.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blotting (1-10ug/ml for affinity pure antibody using ECL technique).
• The antibody will recognize ~34kD Casp-3 as well as the 20-kD, and 18-Kda processed forms of Casp-3.
• A week non-sp band of ~30kD may be detected in some samples
• ELISA: Pure protein can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure)
• Optimal dilution determined by the researcher.
• Anti-human casp-3 cross-react with mouse, rat, bovine, monkey, pig, hamster, and sheep casp-3.
• Purified human Casp-3 can be used as positive control.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.