84-1081-87　Calsarcin-2 (Myozenin-1) 100ul　C1036-04G

特徴

• Calcineurin is a Ca/calmodulin-dependent serine-threonine phosphatase that plays an important role in transducing Ca-dependent signals in a variety of cell types.
• Calcineurin has also been shown to have a profound influence on the properties of striated muscle cells, including cardiac muscle.
• A novel family of striated muscle-specific calcineurin-interacting proteins called calsarcins or Myozenins has been identified that interact and colocalizes with the Z-disc protein alpha-actinin.
• Two isoform of calsarcins, Calsarcin-1 and Calsarcin-2, with specific expression pattern have been identified in human, rat and mouse.
• Calsarcins tether calcineurin to the sarcomere of cardiac and skeletal muscle.
• Besides calcineurin and a-actinin, calsarcins interact with other Z-disc proteins g-filamin, telethonin and TCAP.
• Calsarcin-2/CALS-2 (variouslytermed Myozenin-1 and FATZ) ~34kD, human 299-aa, mouse 296-aa, ~30% identity with CALS-1) is a globular protein with central glycine-rich domain flanked by a-helical regions.
• CALS-2 is expressed transiently in heart during early embryogenesis and later becomes restricted to skeletal muscle with weaker signals in adult prostate, placenta and pancreas.
• In contrast to CALS-1, the expression of Calsarcin-2 is restricted to fast-twitch skeletal fiber (Gastrocnemius), which receives intermittent, high frequency stimulation and exhibit lower intracellular calcium concentration.
• Calsarcin-2 interacts with other Z-disc proteins g-Filamin, ACTN-2, ACTN-3 and TCAP and possibly modulates both g-filamin and a-actinin dimerization.
• Based on its binding partners, Calsarcin-2 has been suggested to play a central role in myofibrillogenesis and as a candidate for limb-girdle muscular dystrophy and other related neuromuscular disorders.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000 (ECL)ELISA: 1:10,000-1:50,000; Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.