84-1081-82　Cachectic Factor (CF) 100ul　C0104-49C

特徴

• Cancer cachexia is a syndrome of progressive wasting which is believed to be mediated by tumor necrosis factor-alpha, interleukins 1 and 6, and interferon-gamma, and leukemia-inhibitory factor.
• However, the levels of these putative agents do not correlate with cachexia.
• Recently, a new circulating tumor factor, termed cachectic factor (CF), has been isolated and characterized from the urine of mice transplanted with adenocarcinoma MAC16 (1).
• Purified CF, a proteoglycan of relative mol.
• Wt of ~24kD, produces cachexia in vivo by inducing catabolism of skeletal muscle.
• CF was also found in urine of cachectic cancer patients.
• It is not found in normal urine, patient with weight loss due to trauma, and cancer patient with no weight loss (1).
• CF appears to be ~24kD on SDS-gels.
• It is a sulfated glycoprotein.
• The amino acid analyses was determined as: YSP EAA SAP GSG DPS HEA.
• An antibody raised to the CF also recognized a 69kD band with the same amino acid sequence as the 24kD material.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-2000 (ECL)ELISA: 1:10-50,000 Control peptide can be used to coat ELISA plates at 1ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.