84-1081-73　Bone Morphogenetic Protein 1 (BMP-1, ProCollagen C-endopeptidase, PCP, mTld, Mammalian Tolloid) 100ug　B2553-03G

特徴

• The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom.
• The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton.
• Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14, other members, such as BMP-3, -6, -7 and –13 may be involved in later stages of skeletal formation.
• BMP1 was first identified in osteogenic bone extracts.
• It is an extracellular zinc endopeptidase implicated in a variety of species.
• BMP1 is expressed at high levels in embryonic maternal deciduum, membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung.
• BMP1 is a shorter spliced variant of mammalian tolloid (mTld) with a 986aa sequence (chr 8p21), both of which cleave the C-propeptides of type I procollagen during the synthesis of extracellular matrix collagen fibrils.
• BMP-1 comprises a metalloproteinase domain, 3 CUB domains, and an EGF-like domain, which is located between the second and third CUB domains.
• It cleaves the C-terminal propeptides of procollagen I, II and III, induces cartilage and bone formation.
• It shows 7 alternatives spliced forms and ubiquitously expressed.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/ml Western Blot: 1-5ug/ml using ECL
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.