Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans.
Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans.
Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-Sheet structure.
Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms.
The genes encoding human alpha- and beta-defensins are clustered in a contiguous segment of chromosome 8p23.
Defensins are classified into two families designated alpha– and beta- based on distinctive, although similar, tri-disulfide linkages in the peptides.
b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides.
In humans, six alpha–defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date.
Rat (RBD-1 and RBD-2) and mouse (MBD1-4) homologs of the human beta-defensin have also been identified.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000; Control peptide (Cat #B0902B) can be used to coat ELISA plates at 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum.
Form：Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes defensin-3. Species Crossreactivity: mouse and human. Does not recognize other beta-defensins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 13aa at the N-terminus of mature mouse defensin-3 and a 15aa peptide mapping at the N-terminus of mature human defensin-3. The two peptide were mixed.
