RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein productsfrom a single structural gene.
Substitutional RNA editing employsa variety of genetic mechanisms, the biochemical basis of whichhas been elucidated following the development of in vitro assaysthat recapitulate important elements of this process.
There are two typesof substitutional RNA exist in mammals, namely A-to-I and C-to-URNA editing.
The best-characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal apolipoprotein B(apo B)).
Apo B RNA editing changes a CAA to a UAA stop codon,generating a truncated protein, apoB48.
The functional complex includes a minimal corecomposed of apobec-1 and ACF, that functionas an adaptor protein by binding both the deaminase and the RNA substrate.
The RNA binding proteins also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2).
ACF (apobec-1 complementation factor) exists in three isoforms; the isoform 3 is a 594aa protein (65kD), when added with apobec-1 it reconstitute the editing of an apo B mRNA template.
It is found to colocalize with apobec-1 and CUGBP2.
It was demonstrated that CUGBP2 co-fractionates with ACF in bovine liver S-100 extracts and that its distribution in the most enriched fractions closely matches with ACF, thus leading to the assembly to apo B holoenzyme.
Applications:Western Blot: 1-10ug/ml using ECL technique
ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Recognizes human ACF.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：17aa peptide from human ACF located at ~C-terminus. Species Sequence Homology: Mouse, rat - 100%.
