84-1081-61　APE (APEX, APEX1, APEX nuclease multifunctional DNA repair enzyme, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, DNA apurinic/apyrimidinic exonuclease/ lyase) 100ul　A2298-74L

特徴

• Reactive oxygen species (H2O2·, OH·, O2, HOCL) generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents can induce a wide variety of lesions in DNA ranging from oxidized bases (8-oxoguanine and thymine glycol), base loss and DNA strand breaks with modified ends such as 3’-phosphate and 3’-phopshoglycolate.
• Unrepaired damaged/modified DNA has been implicated in aging and cancer.
• Many proteins that are involved in BER (base excision repair) have been identified in E coli and mammals.
• Repair of modified is initiated by their removal by specific DNA glycosylase resulting in AP (apurinic/apyrimidinic) sites.
• A series of steps starting with the cleavage of the DNA strand adjacent to AP sites, removal of the deoxyribose phosphate residue, followed by gap filling synthesis and ligation.
• The DNA strand cleavage is catalyzed by a variety of AP-endonucleases (APE or HAP1, APEX, APE-1) that cleave 5’ to the AP sites.
• APE also has an associated 3’-endonuclease activity the removes 3’trans-a,b-unsaturated aldehyde generated by AP lyase, as well as 3’-phosphate and 3’-phosphoglycolate.
• ROS generated 3’-blocked termini at the site of strand breaks are also removed APE.
• Applications:Western Blot: An initial dilution of 1:1K-2K is recommended for Western.
• The antibody has detected ~34kD band from human cells and tissues.
• ELISA: 1:10-50K; using 50-100ng control peptide/well.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.