84-1081-57　Akt1 (Rac PKa, PKBa) 100ul　A1125-01J

特徴

• Putative human homolog of the proto-oncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned.
• These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt.
• RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus.
• The Amino terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins.
• The PH domain (amino acids 1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides.
• The kinase domain is located between residues 148 to 411.
• These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
• AKT1 (RAC-PK-a or PKB-a) is the human homolog of v-akt and is identical to RAC gene.
• The protein has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein.
• Phosphatase treatment has been shown to result in inactivation of the protein.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10ug/ml using Chemiluminescence techniqueELISA: 1:10,000-1:100,000; using 50-100 ng of control peptide/well
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.