84-1081-47　ABCG3 (ATP-binding Cassette (ABC) Transporter 3) 100ug　A0004-04U

特徴

• The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics.
• The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells.
• In addition, increasing numbers of ABC proteins have recently been identified
• ABCG3 protein is a 650aa protein in mouse, highly expressed in the thymus and spleen.
• It seems to have defective ATP binding region, which suggests the ABCG3 protein may not bind or hydrolyze ATP.
• Therefore, it has to dimerize with another subunit to form a functional transporter.
• It is unique among ABC genes in not having several highly conserved residues in the A and C domains of NBF.
• ABCG3 is the most closely related to ABCG2 with 54% homology overall, 64% in the NBF and 50% in the TM region.
• Applications:Suitable for use in ELISA.
• Other potential application, though not tested is Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5-1ug/ml; Use control peptide (Cat #A0004-04U1) to coat ELISA plates at 1ug/ml
• Optimal dilutions to be deermined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.