The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics.
The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells.
In addition, increasing numbers of ABC proteins have recently been identified.
The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules.
ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly.
ABCG2 protein has 655aa in human (Chr 4) and 657 in mouse, is an integral membrane protein, which plays a major role in multidrug resistance phenotype of malignant cells, when overexpresssed.
It appears in three different forms, containing Arg, Gly or Thr at aa position 482, these variants posses significant differences in their cross-resistance and drug transport patterns.
The human ABCG2 protein shows 54% sequence homology with mouse ABCG3 protein.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1-10ug/ml using ECL technique
ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml)
Optimal dilution determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Human control peptide is 100% conserved in Chimp, and 85% in monkey ABCG2, and only 42% in rat and 28% in mouse ABCG2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：14-aa peptide from Human ABCG2 conjugated to KLH ~C-terminus, Extracellular domain
