Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:200- 1:1000Western Blot: 1:20- 1:200Immunohistochemistry: 1:20- 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:goat
Grade:Highly Purified
Purity:Purified IgG fraction to a purity of > 95%.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP)
Specificity:Recognizes numerous Adenovirus proteins including hexon. Does not crossreact with Para 1-3, Infl. A & B, hMPV or Rhinovirus (type 16). Uninfected Cell Reactivity: Negative vs. HE-p2, LLCMK2 and Vero cells by indirect immunofluorescence. Reactive with types 2, 3, 4, 5 & 6. Broad reactivity with most/all Adeno types expected.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Immunogen:Purified, disrupted Adenovirus Type 5 virions.