Direct FA staining of target antigens in a permissive tissue culture system.
Acetone fixation of the antigen source is recommended prior to staining
FLISA: 1:2500.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:goat
Grade:Highly Purified
Purity:Purified IgG fraction to a purity of > 95%.
Form:Supplied as a liquid in PBS, pH 7.2, 10% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:Recognizes Adenovirus. Does not crossreact with Para 1-3, Infl. A & B ,hMPV or Rhinovirus (type 16). Uninfected Cell Reactivity: Negative vs. HE-p2, LLCMK2 and Vero cells by indirect immunofluorescence. Reactive with types 2, 3, 4, 5 & 6. Broad reactivity with most/all Adeno types expected.
Isotype:IgG
Calc Applications Abbrev:FLISA IF
Immunogen:Purified, disrupted Adenovirus Type 5 virions.