Growth differentiation factor 9 (GDF9) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily.
BMP proteins are secreted growth factors that are characterized by seven conserved cysteine residues.
In general, they are regulators of cell growth and differentiation in both embryonic and adult tissues.
GDF9 can form non-covalent homodimers when expressed individually, while when GDF9 and BMP15 are co-expressed BMP-15/GDF-9 heterodimers are produced.
GDF9 signaling might be transduced by binding to the bone morphogenetic protein receptor (BMPR) type II and one or more BMPR type I receptors.
Applications:Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlImmunohistochemistry: 5-15ug/ml will detect GDF9 in cells or tissues
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Growth and Differentiation Factor 9 (GDF9). In direct ELISA, shows <1% crossreactivity with recombinant mouse GDF1, GDF5, GDF6, GDF8 and GDF15. Species Crossreactivity: hamster
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hm Mo
Immunogen：Recombinant protein corresponding to mouse Growth and Differentiation Factor 9 (GDF9) expressed in E. coli.
