Tetracycline is an antibiotic which has the ability to inhibit the growth of wide variety of organisms, including gram-positive and gram-negative bacteria, rickettsias, mycoplasmas, chlamydias, and certain viruses, protozoa, and actinomycetes.
It interferes with the production of proteins that the bacteria need to multiply and divide (bacteriostatic).
Tetracycline Hydrochloride mode of action is as a protein synthesis inhibitor via an aminoacyl-tRNA binding mechanism to the 30S subunit.
Mode of resistance is the loss of cell wall permeability.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:2000Western Blot: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 30% glycerol.
Specificity：Recognizes the TetR protein. Used to monitor the regulation of gene expression in mammalian cells. The TetR antibody is not reactive to human or other mammalian proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Ye
Immunogen：Synthetic peptide corresponding to an internal portion, between aa 25-75, of E. coli TetR protein.
