Applications:Suitable for use in Immunoblot (Western or dot blot), ELISA, Immunohistochemistry and Immunoperoxidase electron microscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:8000, Use ABTS as a substrate for 30 min at RT to assay against 1ug of human IgG.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human IgG Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab’)2.
