The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development.
Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions.
Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notch and related receptors.
JAG1 functions in an embryologically important signaling pathway and has also been shown to play a role in hematopoiesis.
JAG1 inhibits proliferation of cd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravating factor in extrahepatic biliary atresia.
Activation of NOTCH1 signaling by JAG1 induces monocyte-derived dendritic cell maturation in vitro.
JAG1 interaction with NOTCH1 on tumor cells dramatically induces proliferation and inhibition of apoptosis in vitro.
Either haploinsufficiency for wild-type JAG1 and/or dominant negative effects produced by mutated JAG1 are responsible for the Alagille syndrome.
Applications:Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence.
Other applications have not been tested.
Recomnded Dilutions:ELISA: 1:20,000-11:100,000Western Blot: 1:500-1:2000Immunofluorescence: 1:200-1:1000 Immunohistochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity：Recognizes human Jagged1 (JAG1). A band at ~150kD corresponding to Jagged-1 in mouse liver whole cell lysates and a ~75kD band in human brain and kidney lysates. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids near the N-terminus of human Jagged-1 protein. Species Sequence Homology: chimpanzee, rat and mouse; 100%, canine; 81% and Xenopus laevis; 85%.
