Tau is a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules.
Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain.
Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders.
Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons.
Numerous serine/threonine kinases including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, phosphorylate tau.
Microtubule-dependent phosphorylation of threonine 205 is catalyzed by GSK-3b and cdk5 in vitro.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:Recombinant human Tau treated with GSK-3b
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins added. No preservative added. Glycerol-free. Also available with BSA, Azide. See T1032-55.
Specificity：Recognizes human Tau when Tau, phosphorylated at Thr205. Mouse and rat (100% homologous) tau have not been tested, but are expected to react.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from the region of human tau that contains threonine 205. The sequence is conserved in mouse and rat.
